Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein-DNA interactions that occur inside the nucleus of living cells or tissues. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states.
Microarray-based ChIP studies are restricted to a fixed number of probesCurrently, the most common method for genome-wide analysis of DNA-binding proteins is Chip-on-chip (ChIP-chip), which combines chromatin immunoprecipitation with DNA microarrays.
