Whole Genome Shotgun Sequencing of Prokaryotic and Eukaryotic Genomes
The Whole genome sequencing service includes
- Fragmentation by nebulization of the genomic DNA
- Generation and sequencing of non-cloned paired end libraries
- Preparation of a non-cloned single stranded shotgun library (sstDNA) and clonal amplification by emulsion PCR (emPCR)
- Bioinformatics analysis such as ORF calling and annotation
- Closing of gaps by designing primer pairs on adjacent contig ends and sequencing PCR amplicons of the genomicregion of interest
- Subsequent gene synthesis services can be performed to generate clones for specific genes of interest.
- Ultra-high throughput sequencing with GS FLX Titanium-series chemistry up to 20 fold coverage
